Come estrarre DNA da una banana?
Come estrarre DNA da una banana?
Procedimento
- Sbucciare la banana, tagliarla in due e prenderne una metà. Porre la banana in un becher e schiacciarla con una forchetta. ...
- LISI CELLULARE. Si aggiungono 50 mL di acqua, 2 cucchiaini di sale e un cucchiaio di detersivo per i piatti. ...
- ELIMINAZIONE DEI RESIDUI. ...
- PRECIPITAZIONE DEL DNA. ...
- VISUALIZZAZIONE DEL DNA.
Where does IL-6 come from without inflammation?
- In the absence of inflammation 10–35% of circulating IL-6 may come from adipose tissue. IL-6 is produced by adipocytes and is thought to be a reason why obese individuals have higher endogeneous levels of CRP.
What is the role of IL-6 in the nuclear pathway?
- IL-6 activates the Phosphoinositide 3-kinase (PI3K) pathway, and a downstream target of this pathway is the protein kinase B (PKB) (Hodge et al., 2007). IL-6 activated PKB can phosphorylate the nuclear localization signal on DNA methyltransferase-1 (DNMT1). This phosphorylation causes movement of DNMT1 to the nucleus, where it can be transcribed.
How is gene expression regulation for IL-2 regulated?
- Gene expression regulation for IL-2 can be on multiple levels or by different ways. One of the checkpoints is signaling through TCR, antigen receptor of T-lymphocytes after recognizing MHC-peptide complex. Signaling pathway from TCR then goes through phospholipase-C (PLC) dependent pathway.
What is the structure of the IL-2 receptor?
- IL-2 signals through the IL-2 receptor, a complex consisting of three chains, termed alpha (CD25), beta (CD122) and gamma (CD132). The gamma chain is shared by all family members. The IL-2 receptor (IL-2R) α subunit binds IL-2 with low affinity (K d ~ 10 −8 M).
